Journal: International journal of molecular sciences

Article Title: Polydatin Alleviates Cyclophosphamide-Induced Mouse Immunosuppression by Promoting Splenic Lymphocyte Proliferation and Thymic T Cell Development and Differentiation.

doi: 10.3390/ijms26062800

Figure Lengend Snippet: Figure 5. PD regulated the cell cycle and the proliferation-related MAPK/JNK pathway. Represen- tative images of PCNA proteins were tested using a Western blot (WB) (A). Schematic diagram of the effects of CY and PD on cell cycle (B). The cell cycle in splenic lymphocytes was measured and is shown for Normal, CY, CY+PD, and CY+LNT groups (C). Representative images of the Western blot (WB) of phosphorylated MAPK proteins (D). All experiments were performed in triplicate, and molecular weight (KDa) is labeled on the right. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Normal group; # p < 0.05, ## p < 0.01 vs. CY group.

Article Snippet: PD could have activated the cell cycle via activating the MAPK/JNK signaling pathway but we need to pharmacologically inhibit the MAPK/JNK pathway using a specific JNK inhibitor (SP600125, HY-12041, MedChemExpress, Monmouth Junction, NJ, USA) in subsequent stages and compare the proliferation and cell cycle progression of lymphocytes with and without the inhibitor to verify this.

Techniques: Western Blot, Molecular Weight, Labeling

Journal: Frontiers in pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow.

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: FIGURE 8 Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on cyclins D1, E and B1 in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR50 concentrations (at 48 h) of BSHE (see Table 1). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and–treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. *p < 0.05; **p < 0.01.

Article Snippet: Afterwards, samples were incubated overnight at 4°C with different primary antibodies (dilution l:1,000 each) in 5% non-fat dried milk in TBS-T. Primary antibodies were a) from Cell Signaling Technology, Inc, Danvers, MA, United States, i.e., anti-p62/ SQSTM1Ab (cat. 88588), anti-p21 Waf1/Cip1 Ab (cat. BK 2947T), anti-survivin Ab (cat. BK 2808T), anti-p27 Kip1 Ab (cat. BK3698T) and anti-beclin-1 Ab (cat. BK 3495T) b) from Immunological Sciences, Rome, Italy, i.e., anti-cyclin B1 Ab (cat. AB84359), anti-p53 Ab (cat. AB83528), anti-Bcl2 Ab (cat. AB 82346), anti-BAX Ab (cat. 82329) and anti-cyclin D1 Ab (cat. Frontiers in Pharmacology frontiersin.org03 AB82346) c) from Thermo Fisher Scientific Waltham, MA, United States, i.e., anti-cyclin E Ab (cat. MA5-14336) d) from Novus biologicals Littleton, CO, United States, i.e., anti-LC3B Ab (cat. NB100-2220) e) from Santa Cruz Biotechnology, Inc, Santa Cruz, CA, United States, i.e., anti-β-actin Ab (cat. sc47778).

Techniques: Western Blot, Control